Extremely Low Concentrations of Acetic Acid Stimulate Cell Differentiation in Rice Blast Fungus

Misa Kuroki, Yuriko Shiga, Megumi Narukawa-Nara, Takayuki Arazoe, Takashi Kamakura

Research output: Contribution to journalArticle

Abstract

Metabolic switching and rewiring play a dynamic role in programmed cell differentiation. Many pathogenic microbes need to survive in nutrient-deficient conditions and use the glyoxylate cycle, an anaplerotic pathway of the tricarboxylic acid cycle, to produce carbohydrates. The plant pathogenic fungus Magnaporthe oryzae (Pyricularia oryzae) has a unique chitin deacetylase, Cbp1. The spatiotemporal activity of this protein is required for modification of the M. oryzae wall and for cell differentiation into the specialized infection structure (appressorium). Here we show that acetic acid, another product released by the Cbp1-catalyzed conversion of chitin into chitosan, induces appressorium formation. An extremely low concentration (fM) of acetic acid restored cell differentiation in a Δcbp1 mutant possibly through the glyoxylate cycle.

Original languageEnglish
Article number100786
JournaliScience
Volume23
Issue number1
DOIs
Publication statusPublished - 24 Jan 2020

Fingerprint

Magnaporthe oryzae
blast disease
cell differentiation
glyoxylate cycle
acetic acid
appressoria
fungi
chitin deacetylase
tricarboxylic acid cycle
plant pathogenic fungi
chitin
chitosan
carbohydrates
microorganisms
mutants
nutrients
infection
proteins

Keywords

  • Biological Sciences
  • Interaction of Plants with Organisms
  • Molecular Plant Pathology
  • Plant Biology
  • Plant Pathology

Cite this

Kuroki, Misa ; Shiga, Yuriko ; Narukawa-Nara, Megumi ; Arazoe, Takayuki ; Kamakura, Takashi. / Extremely Low Concentrations of Acetic Acid Stimulate Cell Differentiation in Rice Blast Fungus. In: iScience. 2020 ; Vol. 23, No. 1.
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Extremely Low Concentrations of Acetic Acid Stimulate Cell Differentiation in Rice Blast Fungus. / Kuroki, Misa; Shiga, Yuriko; Narukawa-Nara, Megumi; Arazoe, Takayuki; Kamakura, Takashi.

In: iScience, Vol. 23, No. 1, 100786, 24.01.2020.

Research output: Contribution to journalArticle

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