Evidence against requirement of Ser41 and Ser45 for function of PU.1 - Molecular cloning of rat PU.1

Chiharu Nishiyama; Nobutaka Masuoka; Makoto Nishiyama; Tomonobu Ito; Hisakazu Yamane; Ko Okumura; Hideoki Ogawa

doi:10.1016/j.febslet.2004.07.008

Evidence against requirement of Ser41 and Ser45 for function of PU.1 - Molecular cloning of rat PU.1

Chiharu Nishiyama, Nobutaka Masuoka, Makoto Nishiyama, Tomonobu Ito, Hisakazu Yamane, Ko Okumura, Hideoki Ogawa

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The transcription factor PU.1 plays an important role in the development of the myeloid and lymphoid lineages and regulates the transcription of several genes expressed in these cells. Ser41 is conserved in the acidic region (33-47) of PU.1 from a variety of eukaryocytes and has been reported to be one of the two important Ser residues (S41 and S45) for the function of PU.1. In the present study, however, we found that rat PU.1 has Gly at position 41. To elucidate the role of amino acid residues at 41 and 45 in functions of PU.1, we generated mutants of rat PU.1, G41S, G41A, and S45A, and analyzed their transcription-enhancing activities by using two different systems, transient reporter assay system and retroviral transfection system. The amino acid substitution at 41 of PU.1 causes no effect on both transcription-enhancing activity for M-CSF receptor promoter and the cooperative transcription-enhancing activity with GATA-1 for FcεRI α-chain promoter. Furthermore, the substitution at 41 also had no effect on the activity to induce monocyte-specific gene expression in the bone marrow-derived hematopoietic cells. From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter-upregulating function of PU.1.

Original language	English
Pages (from-to)	57-64
Number of pages	8
Journal	FEBS Letters
Volume	572
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.07.008
Publication status	Published - 13 Aug 2004

Keywords

Hematopoiesis
PU.1
Ser
Transcription factor

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10.1016/j.febslet.2004.07.008

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title = "Evidence against requirement of Ser41 and Ser45 for function of PU.1 - Molecular cloning of rat PU.1",

abstract = "The transcription factor PU.1 plays an important role in the development of the myeloid and lymphoid lineages and regulates the transcription of several genes expressed in these cells. Ser41 is conserved in the acidic region (33-47) of PU.1 from a variety of eukaryocytes and has been reported to be one of the two important Ser residues (S41 and S45) for the function of PU.1. In the present study, however, we found that rat PU.1 has Gly at position 41. To elucidate the role of amino acid residues at 41 and 45 in functions of PU.1, we generated mutants of rat PU.1, G41S, G41A, and S45A, and analyzed their transcription-enhancing activities by using two different systems, transient reporter assay system and retroviral transfection system. The amino acid substitution at 41 of PU.1 causes no effect on both transcription-enhancing activity for M-CSF receptor promoter and the cooperative transcription-enhancing activity with GATA-1 for FcεRI α-chain promoter. Furthermore, the substitution at 41 also had no effect on the activity to induce monocyte-specific gene expression in the bone marrow-derived hematopoietic cells. From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter-upregulating function of PU.1.",

keywords = "Hematopoiesis, PU.1, Ser, Transcription factor",

author = "Chiharu Nishiyama and Nobutaka Masuoka and Makoto Nishiyama and Tomonobu Ito and Hisakazu Yamane and Ko Okumura and Hideoki Ogawa",

note = "Funding Information: This work was supported in part by grant-in-aid for Young Scientists from the Ministry of Education, Culture, Sports, Science and Technology of Japan (C.N.). ",

year = "2004",

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doi = "10.1016/j.febslet.2004.07.008",

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T1 - Evidence against requirement of Ser41 and Ser45 for function of PU.1 - Molecular cloning of rat PU.1

AU - Nishiyama, Chiharu

AU - Masuoka, Nobutaka

AU - Nishiyama, Makoto

AU - Ito, Tomonobu

AU - Yamane, Hisakazu

AU - Okumura, Ko

AU - Ogawa, Hideoki

N1 - Funding Information: This work was supported in part by grant-in-aid for Young Scientists from the Ministry of Education, Culture, Sports, Science and Technology of Japan (C.N.).

PY - 2004/8/13

Y1 - 2004/8/13

N2 - The transcription factor PU.1 plays an important role in the development of the myeloid and lymphoid lineages and regulates the transcription of several genes expressed in these cells. Ser41 is conserved in the acidic region (33-47) of PU.1 from a variety of eukaryocytes and has been reported to be one of the two important Ser residues (S41 and S45) for the function of PU.1. In the present study, however, we found that rat PU.1 has Gly at position 41. To elucidate the role of amino acid residues at 41 and 45 in functions of PU.1, we generated mutants of rat PU.1, G41S, G41A, and S45A, and analyzed their transcription-enhancing activities by using two different systems, transient reporter assay system and retroviral transfection system. The amino acid substitution at 41 of PU.1 causes no effect on both transcription-enhancing activity for M-CSF receptor promoter and the cooperative transcription-enhancing activity with GATA-1 for FcεRI α-chain promoter. Furthermore, the substitution at 41 also had no effect on the activity to induce monocyte-specific gene expression in the bone marrow-derived hematopoietic cells. From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter-upregulating function of PU.1.

AB - The transcription factor PU.1 plays an important role in the development of the myeloid and lymphoid lineages and regulates the transcription of several genes expressed in these cells. Ser41 is conserved in the acidic region (33-47) of PU.1 from a variety of eukaryocytes and has been reported to be one of the two important Ser residues (S41 and S45) for the function of PU.1. In the present study, however, we found that rat PU.1 has Gly at position 41. To elucidate the role of amino acid residues at 41 and 45 in functions of PU.1, we generated mutants of rat PU.1, G41S, G41A, and S45A, and analyzed their transcription-enhancing activities by using two different systems, transient reporter assay system and retroviral transfection system. The amino acid substitution at 41 of PU.1 causes no effect on both transcription-enhancing activity for M-CSF receptor promoter and the cooperative transcription-enhancing activity with GATA-1 for FcεRI α-chain promoter. Furthermore, the substitution at 41 also had no effect on the activity to induce monocyte-specific gene expression in the bone marrow-derived hematopoietic cells. From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter-upregulating function of PU.1.

KW - Hematopoiesis

KW - PU.1

KW - Ser

KW - Transcription factor

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Evidence against requirement of Ser41 and Ser45 for function of PU.1 - Molecular cloning of rat PU.1

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