Evaluation of estrogenic activity of wastewater: Comparison among in vitro ERα reporter gene assay, in vivo vitellogenin induction, and chemical analysis

Masaru Ihara, Tomokazu Kitamura, Vimal Kumar, Chang Beom Park, Mariko O. Ihara, Sang Jung Lee, Naoyuki Yamashita, Shinichi Miyagawa, Taisen Iguchi, Seiichiro Okamoto, Yutaka Suzuki, Hiroaki Tanaka

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20 Citations (Scopus)

Abstract

The in vitro estrogen receptor (ER) reporter gene assay has long been used to measure estrogenic activity in wastewater. In a previous study, we demonstrated that the assay represents net estrogenic activity in the balance between estrogenic and antiestrogenic activities in wastewater. However, it remained unclear whether the net estrogenic activity measured by the in vitro ERα reporter gene assay can predict the in vivo estrogenic effect of wastewater. To determine this, we measured the following: estrogenic and antiestrogenic activities of wastewater and reclaimed water by the in vitro ERα reporter gene assay, expression of vitellogenin-1 (vtg1) and choriogenin-H (chgH) in male medaka (Oryzias latipes) by quantitative real-time PCR, and estrone, 17β-estradiol, estriol, and 17α-ethynylestradiol concentrations chemically to predict estrogenic activity. The net estrogenic activity measured by the in vitro medaka ERα reporter gene assay predicted the in vivo vtg1/chgH expression in male medaka more accurately than the concentrations of estrogens. These results also mean that in vivo vtg1/chgH expression in male medaka is determined by the balance between estrogenic and antiestrogenic activities. The in vitro medaka ERα reporter gene assay also predicted in vivo vtg1/chgH expression on male medaka better than the human ERα reporter gene assay.

Original languageEnglish
Pages (from-to)6319-6326
Number of pages8
JournalEnvironmental Science and Technology
Volume49
Issue number10
DOIs
Publication statusPublished - 19 May 2015

Cite this

Ihara, M., Kitamura, T., Kumar, V., Park, C. B., Ihara, M. O., Lee, S. J., Yamashita, N., Miyagawa, S., Iguchi, T., Okamoto, S., Suzuki, Y., & Tanaka, H. (2015). Evaluation of estrogenic activity of wastewater: Comparison among in vitro ERα reporter gene assay, in vivo vitellogenin induction, and chemical analysis. Environmental Science and Technology, 49(10), 6319-6326. https://doi.org/10.1021/acs.est.5b01027