Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

Masanori Nishi; Jun Kobayashi; Masayuki Yamato; Akihiko Kikuchi; Katsumi Uchida; Hirofumi Yajima; Teruo Okano

Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

Masanori Nishi
, Jun Kobayashi
, Masayuki Yamato
, Akihiko Kikuchi
, Katsumi Uchida
, Hirofumi Yajima
, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G₁₂RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G₁₂RGDS. Consequently, thermoresponsive TCPS modified with G₁₂RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

Original language	English
Pages	5071-5072
Number of pages	2
Publication status	Published - 1 Dec 2006
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 20 Sept 2006 → 22 Sept 2006

Conference

Conference	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	20/09/06 → 22/09/06

Keywords

2-carboxyisopropylacrylamide
Avidin
Biotin
Cell culture
Electron beam irradiation
Thermoresponsive surface

Cite this

@conference{736dbb0cf9704d2d88215d59ef9acd8c,

title = "Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding",

abstract = "we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.",

keywords = "2-carboxyisopropylacrylamide, Avidin, Biotin, Cell culture, Electron beam irradiation, Thermoresponsive surface",

author = "Masanori Nishi and Jun Kobayashi and Masayuki Yamato and Akihiko Kikuchi and Katsumi Uchida and Hirofumi Yajima and Teruo Okano",

year = "2006",

month = dec,

day = "1",

language = "English",

pages = "5071--5072",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

}

TY - CONF

T1 - Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

AU - Nishi, Masanori

AU - Kobayashi, Jun

AU - Yamato, Masayuki

AU - Kikuchi, Akihiko

AU - Uchida, Katsumi

AU - Yajima, Hirofumi

AU - Okano, Teruo

PY - 2006/12/1

Y1 - 2006/12/1

N2 - we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

AB - we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

KW - 2-carboxyisopropylacrylamide

KW - Avidin

KW - Biotin

KW - Cell culture

KW - Electron beam irradiation

KW - Thermoresponsive surface

UR - https://www.scopus.com/pages/publications/33846166788

M3 - Paper

AN - SCOPUS:33846166788

SP - 5071

EP - 5072

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

Abstract

Conference

Keywords

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