Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

Masanori Nishi; Jun Kobayashi; Masayuki Yamato; Akihiko Kikuchi; Katsumi Uchida; Hirofumi Yajima; Teruo Okano

Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

Masanori Nishi, Jun Kobayashi, Masayuki Yamato, Akihiko Kikuchi, Katsumi Uchida, Hirofumi Yajima, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G₁₂RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G₁₂RGDS. Consequently, thermoresponsive TCPS modified with G₁₂RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

Original language	English
Pages	5071-5072
Number of pages	2
Publication status	Published - 1 Dec 2006
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 20 Sept 2006 → 22 Sept 2006

Conference

Conference	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	20/09/06 → 22/09/06

Keywords

2-carboxyisopropylacrylamide
Avidin
Biotin
Cell culture
Electron beam irradiation
Thermoresponsive surface

Cite this

@conference{736dbb0cf9704d2d88215d59ef9acd8c,

title = "Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding",

abstract = "we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.",

keywords = "2-carboxyisopropylacrylamide, Avidin, Biotin, Cell culture, Electron beam irradiation, Thermoresponsive surface",

author = "Masanori Nishi and Jun Kobayashi and Masayuki Yamato and Akihiko Kikuchi and Katsumi Uchida and Hirofumi Yajima and Teruo Okano",

year = "2006",

month = dec,

day = "1",

language = "English",

pages = "5071--5072",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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TY - CONF

T1 - Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

AU - Nishi, Masanori

AU - Kobayashi, Jun

AU - Yamato, Masayuki

AU - Kikuchi, Akihiko

AU - Uchida, Katsumi

AU - Yajima, Hirofumi

AU - Okano, Teruo

PY - 2006/12/1

Y1 - 2006/12/1

N2 - we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

AB - we developed a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(IPAAm-co-CIPAAm)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37 °C. Moreover, adhered HUVECs were detached after lowering temperature to 20 °C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptides allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

KW - 2-carboxyisopropylacrylamide

KW - Avidin

KW - Biotin

KW - Cell culture

KW - Electron beam irradiation

KW - Thermoresponsive surface

UR - http://www.scopus.com/inward/record.url?scp=33846166788&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33846166788

SP - 5071

EP - 5072

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Development of thermoresponsive culture dishes modified with bioactive molecules by affinity binding

Abstract

Conference

Keywords

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