Copper, nickel and zinc complexes of 3-acetyl coumarin thiosemicarbazone: Synthesis, characterization and in vitro evaluation of cytotoxicity and DNA/protein binding properties

K. N.Anees Rahman, Jebiti Haribabu, Chandrasekar Balachandran, Nattamai S.P. Bhuvanesh, Ramasamy Karvembu, Anandram Sreekanth

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57 Citations (Scopus)

Abstract

A new 3-acetylcoumarin thiosemicarbazone (AcTsc) and its copper(II) (1), nickel(II) (2) and zinc(II) (3) complexes were synthesized and characterized by elemental analysis, UV–Vis, FT-IR, 1H and 13C NMR/EPR and mass spectroscopic techniques. The molecular structure of AcTsc, and the complexes 1 and 3 was confirmed by single crystal X-ray crystallography. The interaction of the complexes (1–3) with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) was explored using absorption and emission spectral methods, and viscosity measurement. The spectroscopic results clearly suggested that the complexes (1–3) interacted with CT-DNA through intercalative binding mode. In addition, all the complexes were subjected to cytotoxic studies against human liver carcinoma (HepG-2), lung carcinoma (A549), human leukemic monocyte lymphoma (U937) and lymphoblastoid multiple myeloma cells (IM-9). Complex 3 showed significant cytotoxicity against human liver carcinoma (HepG-2) and lymphoblastoid multiple myeloma (IM-9) cell lines with the IC50 value of 25 μg/mL.

Original languageEnglish
Pages (from-to)26-35
Number of pages10
JournalPolyhedron
Volume135
DOIs
Publication statusPublished - 2017

Keywords

  • Copper(II)
  • Cytotoxicity
  • DNA/BSA interaction
  • Nickel(II)
  • Zinc(II)

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