Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Kazuhiro Fukumori; Shintaroh Iwanaga; Yoshikatsu Akiyama; Akihiko Kikuchi; Masayuki Yamato; Kiyotaka Sakai; Teruo Okano

Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Kazuhiro Fukumori, Shintaroh Iwanaga, Yoshikatsu Akiyama, Akihiko Kikuchi, Masayuki Yamato, Kiyotaka Sakai, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesftilly grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at 30wt% concentratioa These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted soverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.

Original language	English
Number of pages	1
Publication status	Published - 1 Dec 2005
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 25 May 2005 → 27 May 2005

Conference

Conference	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	25/05/05 → 27/05/05

Keywords

Cell sheet
Electron beam
N-isopropylacrylamide
Thermoresponsive surface

Cite this

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title = "Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation",

abstract = "PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesftilly grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at 30wt% concentratioa These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted soverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.",

keywords = "Cell sheet, Electron beam, N-isopropylacrylamide, Thermoresponsive surface",

author = "Kazuhiro Fukumori and Shintaroh Iwanaga and Yoshikatsu Akiyama and Akihiko Kikuchi and Masayuki Yamato and Kiyotaka Sakai and Teruo Okano",

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day = "1",

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note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

AU - Fukumori, Kazuhiro

AU - Iwanaga, Shintaroh

AU - Akiyama, Yoshikatsu

AU - Kikuchi, Akihiko

AU - Yamato, Masayuki

AU - Sakai, Kiyotaka

AU - Okano, Teruo

PY - 2005/12/1

Y1 - 2005/12/1

N2 - PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesftilly grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at 30wt% concentratioa These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted soverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.

AB - PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesftilly grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at 30wt% concentratioa These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted soverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.

KW - Cell sheet

KW - Electron beam

KW - N-isopropylacrylamide

KW - Thermoresponsive surface

UR - http://www.scopus.com/inward/record.url?scp=33645641442&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645641442

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Abstract

Conference

Keywords

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