Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Kazuhiro Fukumori; Shintaroh Iwanaga; Yoshikatsu Akiyama; Akihiko Kikuchi; Masayuki Yamato; Kiyotaka Sakai; Temo Okano

Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Kazuhiro Fukumori, Shintaroh Iwanaga, Yoshikatsu Akiyama, Akihiko Kikuchi, Masayuki Yamato, Kiyotaka Sakai, Temo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at more than 40wt% concentration These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted coverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.

Original language	English
Pages	5221-5222
Number of pages	2
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 20 Sept 2005 → 22 Sept 2005

Conference

Conference	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	20/09/05 → 22/09/05

Keywords

Cell sheet
Electron beam
N-isopropy laciy lamide
Thermoiesponsive surface

Cite this

@conference{1cd022b85d044bcebaf25ad97e711a5c,

title = "Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation",

abstract = "PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at more than 40wt% concentration These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted coverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.",

keywords = "Cell sheet, Electron beam, N-isopropy laciy lamide, Thermoiesponsive surface",

author = "Kazuhiro Fukumori and Shintaroh Iwanaga and Yoshikatsu Akiyama and Akihiko Kikuchi and Masayuki Yamato and Kiyotaka Sakai and Temo Okano",

year = "2005",

language = "English",

pages = "5221--5222",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

}

TY - CONF

T1 - Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

AU - Fukumori, Kazuhiro

AU - Iwanaga, Shintaroh

AU - Akiyama, Yoshikatsu

AU - Kikuchi, Akihiko

AU - Yamato, Masayuki

AU - Sakai, Kiyotaka

AU - Okano, Temo

PY - 2005

Y1 - 2005

N2 - PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at more than 40wt% concentration These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted coverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.

AB - PIPAAm grafted coverslip was prepared using electron beam. ESCA analysis of resulting surfaces revealed that PIPAAm was succesfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. PIPAAm grafted coverslips prepared at 5wt% monomer concentration showed cell attachment and detachment properties by temperature changes. However, cells did not adhere to PIPAAm grafted coverslips prepared at more than 40wt% concentration These results indicate that 5wt% monomer concentration should be appropriate condition for preparation of PIPAAm grafted coverslips exhibiting the intelligent properties, hi addition, the results also suggest that EB irradiation is convenient method for grafting polymers onto inorganic surfaces such glass surfaces.

KW - Cell sheet

KW - Electron beam

KW - N-isopropy laciy lamide

KW - Thermoiesponsive surface

UR - http://www.scopus.com/inward/record.url?scp=33645559663&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645559663

SP - 5221

EP - 5222

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Abstract

Conference

Keywords

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