Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Kazuhiro Fukumori; Shintaroh Iwanaga; Yoshikatsu Akiyama; Akihiko Kikuchi; Masayuki Yamato; Kiyotaka Sakai; Teruo Okano

Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Kazuhiro Fukumori, Shintaroh Iwanaga, Yoshikatsu Akiyama, Akihiko Kikuchi, Masayuki Yamato, Kiyotaka Sakai, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

PIPAAm was grafted onto coverslips modified with MPTMS, using EB irradiation. ESCA analysis of resulting surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. Cells adhered to the surfaces of 4.0 nm thickness of the grafted PIPAAm layer. By contrast, PIPAAm layer with 8.0 nm thickness exhibited cell repellent surfaces. This tendency is similar to PIPAAm grafted TCPS in our previous report, although the grafted layer is extremely thinner. We discuss an influence of thickness of the grafted PIPAAm onto cell adhesion / detachment properties, comparing to PIAPAm grafted TCPS in the previous report.

Original language	English
Pages	1977
Number of pages	1
Publication status	Published - 2006
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 24 May 2006 → 26 May 2006

Conference

Conference	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	24/05/06 → 26/05/06

Keywords

Electron beam irradiation
N-isopropylacrylamide
Thermoresponsive surface
Total internal reflection microscopy

Cite this

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title = "Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation",

abstract = "PIPAAm was grafted onto coverslips modified with MPTMS, using EB irradiation. ESCA analysis of resulting surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. Cells adhered to the surfaces of 4.0 nm thickness of the grafted PIPAAm layer. By contrast, PIPAAm layer with 8.0 nm thickness exhibited cell repellent surfaces. This tendency is similar to PIPAAm grafted TCPS in our previous report, although the grafted layer is extremely thinner. We discuss an influence of thickness of the grafted PIPAAm onto cell adhesion / detachment properties, comparing to PIAPAm grafted TCPS in the previous report.",

keywords = "Electron beam irradiation, N-isopropylacrylamide, Thermoresponsive surface, Total internal reflection microscopy",

author = "Kazuhiro Fukumori and Shintaroh Iwanaga and Yoshikatsu Akiyama and Akihiko Kikuchi and Masayuki Yamato and Kiyotaka Sakai and Teruo Okano",

year = "2006",

language = "English",

pages = "1977",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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TY - CONF

T1 - Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

AU - Fukumori, Kazuhiro

AU - Iwanaga, Shintaroh

AU - Akiyama, Yoshikatsu

AU - Kikuchi, Akihiko

AU - Yamato, Masayuki

AU - Sakai, Kiyotaka

AU - Okano, Teruo

PY - 2006

Y1 - 2006

N2 - PIPAAm was grafted onto coverslips modified with MPTMS, using EB irradiation. ESCA analysis of resulting surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. Cells adhered to the surfaces of 4.0 nm thickness of the grafted PIPAAm layer. By contrast, PIPAAm layer with 8.0 nm thickness exhibited cell repellent surfaces. This tendency is similar to PIPAAm grafted TCPS in our previous report, although the grafted layer is extremely thinner. We discuss an influence of thickness of the grafted PIPAAm onto cell adhesion / detachment properties, comparing to PIAPAm grafted TCPS in the previous report.

AB - PIPAAm was grafted onto coverslips modified with MPTMS, using EB irradiation. ESCA analysis of resulting surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. Cells adhered to the surfaces of 4.0 nm thickness of the grafted PIPAAm layer. By contrast, PIPAAm layer with 8.0 nm thickness exhibited cell repellent surfaces. This tendency is similar to PIPAAm grafted TCPS in our previous report, although the grafted layer is extremely thinner. We discuss an influence of thickness of the grafted PIPAAm onto cell adhesion / detachment properties, comparing to PIAPAm grafted TCPS in the previous report.

KW - Electron beam irradiation

KW - N-isopropylacrylamide

KW - Thermoresponsive surface

KW - Total internal reflection microscopy

UR - http://www.scopus.com/inward/record.url?scp=33749860730&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749860730

SP - 1977

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation

Abstract

Conference

Keywords

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