Continuous sweat lactate monitoring system with integrated screen-printed Mgo-templated carbon-lactate oxidase biosensor and microfluidic sweat collector

Isao Shitanda, Masaya Mitsumoto, Noya Loew, Yuko Yoshihara, Hikari Watanabe, Tsutomu Mikawa, Seiya Tsujimura, Masayuki Itagaki, Masahiro Motosuke

Research output: Contribution to journalArticlepeer-review

Abstract

The continuous monitoring of sweat lactate enables the real-time detection of the lactate threshold, that is, the change from aerobic to anaerobic metabolism. Therefore, it is important to optimize the training of athletes and physical exercise of rehabilitation patients and the elderly and to control the exertion of high-performance workers such as firefighters. In this study, we developed a lactate sensing system using a screen-printed sensor based on graft-polymerized MgO-templated carbon onto which lactate oxidase and 1,2-naphthoquinone were immobilized. Additionally, the lactate sensing system features an integrated microfluidic sweat collector fabricated from polydimethylsiloxane. With a lactate detection limit of 0.3 mM and a dynamic range of up to 50 mM, the developed system is suitable for the detection of the lactate threshold. Moreover, the lactate sensing system developed in this study is lightweight, should be comfortable to wear on the skin, and exhibits a sensing performance that is adequate for the continuous monitoring of sweat lactate.

Original languageEnglish
Article number137620
JournalElectrochimica Acta
Volume368
DOIs
Publication statusPublished - 1 Feb 2021

Keywords

  • Continuous sweat lactate monitoring
  • Graft polymerization
  • Lactate oxidase
  • Mesoporous carbon
  • Microfluidics

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