@article{1858922ef2b840ab84a0d2f58802be7f,
title = "Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging",
abstract = "This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.",
keywords = "computed tomography, fluorescence, near infrared, three-dimensional imaging",
author = "Masakazu Umezawa and Toshihiro Sera and Hideo Yokota and Maho Takematsu and Masahiko Morita and Gil Yeroslavsky and Masao Kamimura and Kohei Soga",
note = "Funding Information: We thank the graduate and undergraduate students for their technical assistance in the Soga Laboratory in Tokyo University of Science, especially Mr. Soichiro Fujii and Mr. Takeaki Harada for optical system setup, Mr. Ryuta Takeda and Mr. Takaya Yamada for preparation of NaGdF co‐doped with Yb and Ho, and Ms. Yuki Uenomachi for optimizing agarose gel‐based biological phantom. This work was supported in part by the MEXT Grant‐in‐Aid for Scientific Research on Innovative Areas (Resonance Bio), no. 15H05950, the MEXT Grant‐in‐Aid for Scientific Research (A), no. 19H01179, the MEXT‐Supported Program for the Strategic Research Foundation at Private Universities, no. S1511012, and the Center of Innovation Program “COINS” from Japan Science and Technology Agency, JST. 4 3+ 3+ Funding Information: Ministry of Education, Culture, Sports, Science and Technology, Grant/Award Numbers: S1511012, 19H01179, 15H05950; Japan Science and Technology Agency, Grant/Award Number: Center of Innovation Program “COINS” Funding information Funding Information: We thank the graduate and undergraduate students for their technical assistance in the Soga Laboratory in Tokyo University of Science, especially Mr. Soichiro Fujii and Mr. Takeaki Harada for optical system setup, Mr. Ryuta Takeda and Mr. Takaya Yamada for preparation of NaGdF4 co-doped with Yb3+ and Ho3+, and Ms. Yuki Uenomachi for optimizing agarose gel-based biological phantom. This work was supported in part by the MEXT Grant-in-Aid for Scientific Research on Innovative Areas (Resonance Bio), no. 15H05950, the MEXT Grant-in-Aid for Scientific Research (A), no. 19H01179, the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, no. S1511012, and the Center of Innovation Program ?COINS? from Japan Science and Technology Agency, JST. Publisher Copyright: {\textcopyright} 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2020",
month = aug,
day = "1",
doi = "10.1002/jbio.202000071",
language = "English",
volume = "13",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "8",
}