Commensal bacteria directly suppress in vitro degranulation of mast cells in a MyD88-independent manner

Kazumi Kasakura; Kyoko Takahashi; Tomoko Itoh; Akira Hosono; Yoshika Momose; Kikuji Itoh; Chiharu Nishiyama; Shuichi Kaminogawa

doi:10.1080/09168451.2014.930327

Commensal bacteria directly suppress in vitro degranulation of mast cells in a MyD88-independent manner

Kazumi Kasakura, Kyoko Takahashi, Tomoko Itoh, Akira Hosono, Yoshika Momose, Kikuji Itoh, Chiharu Nishiyama, Shuichi Kaminogawa

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. Epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. However, the cellular and molecular mechanisms underlying these effects remain to be determined. Here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a MyD88-independent manner. In particular, Enterococcus faecalis showed the strongest suppression of degranulation through partial inhibition of Ca²⁺ signaling upon the high affinity IgE receptor (FcεRI) cross-linking.

Original language	English
Pages (from-to)	1669-1676
Number of pages	8
Journal	Bioscience, Biotechnology and Biochemistry
Volume	78
Issue number	10
DOIs	https://doi.org/10.1080/09168451.2014.930327
Publication status	Published - 2014

Keywords

Allergy
Commensal bacteria
Degranulation
Mast cells

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10.1080/09168451.2014.930327

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title = "Commensal bacteria directly suppress in vitro degranulation of mast cells in a MyD88-independent manner",

abstract = "The intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. Epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. However, the cellular and molecular mechanisms underlying these effects remain to be determined. Here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a MyD88-independent manner. In particular, Enterococcus faecalis showed the strongest suppression of degranulation through partial inhibition of Ca2+ signaling upon the high affinity IgE receptor (FcεRI) cross-linking.",

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author = "Kazumi Kasakura and Kyoko Takahashi and Tomoko Itoh and Akira Hosono and Yoshika Momose and Kikuji Itoh and Chiharu Nishiyama and Shuichi Kaminogawa",

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T1 - Commensal bacteria directly suppress in vitro degranulation of mast cells in a MyD88-independent manner

AU - Kasakura, Kazumi

AU - Takahashi, Kyoko

AU - Itoh, Tomoko

AU - Hosono, Akira

AU - Momose, Yoshika

AU - Itoh, Kikuji

AU - Nishiyama, Chiharu

AU - Kaminogawa, Shuichi

PY - 2014

Y1 - 2014

N2 - The intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. Epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. However, the cellular and molecular mechanisms underlying these effects remain to be determined. Here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a MyD88-independent manner. In particular, Enterococcus faecalis showed the strongest suppression of degranulation through partial inhibition of Ca2+ signaling upon the high affinity IgE receptor (FcεRI) cross-linking.

AB - The intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. Epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. However, the cellular and molecular mechanisms underlying these effects remain to be determined. Here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a MyD88-independent manner. In particular, Enterococcus faecalis showed the strongest suppression of degranulation through partial inhibition of Ca2+ signaling upon the high affinity IgE receptor (FcεRI) cross-linking.

KW - Allergy

KW - Commensal bacteria

KW - Degranulation

KW - Mast cells

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SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

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ER -

Commensal bacteria directly suppress in vitro degranulation of mast cells in a MyD88-independent manner

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Keywords

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