Cloning of full-length genomic DNA encoding human FcεRI α-chain and its transcriptional regulation

Chiharu Nishiyama; Masanari Hasegawa; Makoto Nishiyama; Kyoko Takahashi; Toyokazu Yokota; Ko Okumura; Chisei Ra

doi:10.1006/bbrc.2001.5079

Cloning of full-length genomic DNA encoding human FcεRI α-chain and its transcriptional regulation

Chiharu Nishiyama, Masanari Hasegawa, Makoto Nishiyama, Kyoko Takahashi, Toyokazu Yokota, Ko Okumura, Chisei Ra

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human FcεRI α-chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the "first intron" between exon 1A and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcεRI α-chain positive cells.

Original language	English
Pages (from-to)	1056-1064
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	284
Issue number	4
DOIs	https://doi.org/10.1006/bbrc.2001.5079
Publication status	Published - 2001

Keywords

FcεRI α-chain
Genomic DNA
Promoter

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10.1006/bbrc.2001.5079

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abstract = "Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human FcεRI α-chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the {"}first intron{"} between exon 1A and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcεRI α-chain positive cells.",

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author = "Chiharu Nishiyama and Masanari Hasegawa and Makoto Nishiyama and Kyoko Takahashi and Toyokazu Yokota and Ko Okumura and Chisei Ra",

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T1 - Cloning of full-length genomic DNA encoding human FcεRI α-chain and its transcriptional regulation

AU - Nishiyama, Chiharu

AU - Hasegawa, Masanari

AU - Nishiyama, Makoto

AU - Takahashi, Kyoko

AU - Yokota, Toyokazu

AU - Okumura, Ko

AU - Ra, Chisei

PY - 2001

Y1 - 2001

N2 - Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human FcεRI α-chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the "first intron" between exon 1A and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcεRI α-chain positive cells.

AB - Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human FcεRI α-chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the "first intron" between exon 1A and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcεRI α-chain positive cells.

KW - FcεRI α-chain

KW - Genomic DNA

KW - Promoter

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EP - 1064

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Cloning of full-length genomic DNA encoding human FcεRI α-chain and its transcriptional regulation

Abstract

Keywords

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