Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus

Akihito Nozaka, Ayaka Nishiwaki, Yuka Nagashima, Shogo Endo, Misa Kuroki, Masahiro Nakajima, Megumi Narukawa, Shinji Kamisuki, Takayuki Arazoe, Hayao Taguchi, Fumio Sugawara, Takashi Kamakura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chloramphenicol (Cm) is a broad-spectrum classic antibiotic active against prokaryotic organisms. However, Cm has severe side effects in eukaryotes of which the cause remains unknown. The plant pathogenic fungus Magnaporthe oryzae, which causes rice blast, forms an appressorium to infect the host cell via single-cell differentiation. Chloramphenicol specifically inhibits appressorium formation, which indicates that Cm has a novel molecular target (or targets) in the rice blast fungus. Application of the T7 phage display method inferred that MoDullard, a Ser/Thr-protein phosphatase, may be a target of Cm. In animals Dullard functions in cell differentiation and protein synthesis, but in fungi its role is poorly understood. In vivo and in vitro analyses showed that MoDullard is required for appressorium formation, and that Cm can bind to and inhibit MoDullard function. Given that human phosphatase CTDSP1 complemented the MoDullard function during appressorium formation by M. oryzae, CTDSP1 may be a novel molecular target of Cm in eukaryotes.

Original languageEnglish
Article number9283
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Chloramphenicol
Phosphoric Monoester Hydrolases
Cell Differentiation
Fungi
Infection
Eukaryota
Magnaporthe
Bacteriophage T7
Phosphoprotein Phosphatases
Oryza
Anti-Bacterial Agents
Proteins

Cite this

Nozaka, Akihito ; Nishiwaki, Ayaka ; Nagashima, Yuka ; Endo, Shogo ; Kuroki, Misa ; Nakajima, Masahiro ; Narukawa, Megumi ; Kamisuki, Shinji ; Arazoe, Takayuki ; Taguchi, Hayao ; Sugawara, Fumio ; Kamakura, Takashi. / Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus. / Nozaka, Akihito; Nishiwaki, Ayaka; Nagashima, Yuka; Endo, Shogo; Kuroki, Misa; Nakajima, Masahiro; Narukawa, Megumi; Kamisuki, Shinji; Arazoe, Takayuki; Taguchi, Hayao; Sugawara, Fumio; Kamakura, Takashi.

In: Scientific reports, Vol. 9, No. 1, 9283, 01.12.2019.

Research output: Contribution to journalArticle

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AU - Nishiwaki, Ayaka

AU - Nagashima, Yuka

AU - Endo, Shogo

AU - Kuroki, Misa

AU - Nakajima, Masahiro

AU - Narukawa, Megumi

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AU - Arazoe, Takayuki

AU - Taguchi, Hayao

AU - Sugawara, Fumio

AU - Kamakura, Takashi

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