Cell-array preparation onto the surfaces having various surface characters by photolithography technique

Akihiro Hirano; Masahiro Ichino; Takeshi Ikeya; Tohru Shibuya; Hidenori Otsuka; Satoshi Suzuki; Shin Enosawa; Yukio Nagasaki; Kazunori Kataoka

Cell-array preparation onto the surfaces having various surface characters by photolithography technique

Akihiro Hirano, Masahiro Ichino, Takeshi Ikeya, Tohru Shibuya, Hidenori Otsuka, Satoshi Suzuki, Shin Enosawa, Yukio Nagasaki, Kazunori Kataoka

Department of Applied Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

In this study, two kind of water-soluble photoreactive polymers were utilized for cell patterning. These polymers were spincoated onto the substrate covered with collagen. The substrates were then irradiated by the UV light (365 nm) through the quartz photomask. After water development, micropatterned- polymeric surfaces were obtained. Bovine aortic endothelial cells were seeded onto the surface and all of the cells were adhered only onto the collagen-exposure domains. The results of hepatocyte culture onto the surface, Az-8-PEG is rather effective for inhibition of non-specific adhesion of the hepatocytes. Cells culture on collagen(+) micropatterned surface were maintained for a long period.

Original language	English
Pages	4851-4852
Number of pages	2
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 20 Sept 2005 → 22 Sept 2005

Conference

Conference	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	20/09/05 → 22/09/05

Keywords

Cell array
Micro patterning
Photoresist
Poly (ethylene glycol)
Poly (vinyl alcohol)
Surface modification

Cite this

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title = "Cell-array preparation onto the surfaces having various surface characters by photolithography technique",

abstract = "In this study, two kind of water-soluble photoreactive polymers were utilized for cell patterning. These polymers were spincoated onto the substrate covered with collagen. The substrates were then irradiated by the UV light (365 nm) through the quartz photomask. After water development, micropatterned- polymeric surfaces were obtained. Bovine aortic endothelial cells were seeded onto the surface and all of the cells were adhered only onto the collagen-exposure domains. The results of hepatocyte culture onto the surface, Az-8-PEG is rather effective for inhibition of non-specific adhesion of the hepatocytes. Cells culture on collagen(+) micropatterned surface were maintained for a long period.",

keywords = "Cell array, Micro patterning, Photoresist, Poly (ethylene glycol), Poly (vinyl alcohol), Surface modification",

author = "Akihiro Hirano and Masahiro Ichino and Takeshi Ikeya and Tohru Shibuya and Hidenori Otsuka and Satoshi Suzuki and Shin Enosawa and Yukio Nagasaki and Kazunori Kataoka",

year = "2005",

language = "English",

pages = "4851--4852",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

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TY - CONF

T1 - Cell-array preparation onto the surfaces having various surface characters by photolithography technique

AU - Hirano, Akihiro

AU - Ichino, Masahiro

AU - Ikeya, Takeshi

AU - Shibuya, Tohru

AU - Otsuka, Hidenori

AU - Suzuki, Satoshi

AU - Enosawa, Shin

AU - Nagasaki, Yukio

AU - Kataoka, Kazunori

PY - 2005

Y1 - 2005

N2 - In this study, two kind of water-soluble photoreactive polymers were utilized for cell patterning. These polymers were spincoated onto the substrate covered with collagen. The substrates were then irradiated by the UV light (365 nm) through the quartz photomask. After water development, micropatterned- polymeric surfaces were obtained. Bovine aortic endothelial cells were seeded onto the surface and all of the cells were adhered only onto the collagen-exposure domains. The results of hepatocyte culture onto the surface, Az-8-PEG is rather effective for inhibition of non-specific adhesion of the hepatocytes. Cells culture on collagen(+) micropatterned surface were maintained for a long period.

AB - In this study, two kind of water-soluble photoreactive polymers were utilized for cell patterning. These polymers were spincoated onto the substrate covered with collagen. The substrates were then irradiated by the UV light (365 nm) through the quartz photomask. After water development, micropatterned- polymeric surfaces were obtained. Bovine aortic endothelial cells were seeded onto the surface and all of the cells were adhered only onto the collagen-exposure domains. The results of hepatocyte culture onto the surface, Az-8-PEG is rather effective for inhibition of non-specific adhesion of the hepatocytes. Cells culture on collagen(+) micropatterned surface were maintained for a long period.

KW - Cell array

KW - Micro patterning

KW - Photoresist

KW - Poly (ethylene glycol)

KW - Poly (vinyl alcohol)

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=33645569860&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645569860

SP - 4851

EP - 4852

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Cell-array preparation onto the surfaces having various surface characters by photolithography technique

Abstract

Conference

Keywords

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