Bioengineered vascularized myocardial tissue by multi-step transplantation of layered cell sheets

T. Shimizu, Y. Isoi, H. Sekine, M. Yamato, Akihiko Kikuchi, T. Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The fabrication of three-dimensional (3-D) tissue by layering cell sheets without any biodegradable scaffolds was analyzed. Tissue culture polystyrene dishes were grafted with temperature-response polymer, poly(N-ispropylacryamide) (PIPAAm) by electron beam. Cardiomyocytes were isolated from neonatal rats with the collagenase and cultured confluently on PIPAAm-grafted surfaces at 37°C. The results show that the limitation of engineered-tissue thickness due to primary hypoxia can be dissolved by cell sheets technology and multi-transplantation technique.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 1 Dec 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Conference

ConferenceTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period17/05/0421/05/04

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Shimizu, T., Isoi, Y., Sekine, H., Yamato, M., Kikuchi, A., & Okano, T. (2004). Bioengineered vascularized myocardial tissue by multi-step transplantation of layered cell sheets. In Transactions - 7th World Biomaterials Congress (Transactions - 7th World Biomaterials Congress).