Arabidopsis TBP-ASSOCIATED FACTOR 12 ortholog NOBIRO6 controls root elongation with unfolded protein response cofactor activity

June Sik Kim, Yuki Sakamoto, Fuminori Takahashi, Michitaro Shibata, Kaoru Urano, Sachihiro Matsunaga, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

Research output: Contribution to journalArticlepeer-review

Abstract

Plant root growth is indeterminate but continuously responds to environmental changes. We previously reported on the severe root growth defect of a double mutant in bZIP17 and bZIP28 (bz1728) modulating the unfolded protein response (UPR). To elucidate the mechanism by which bz1728 seedlings develop a short root, we obtained a series of bz1728 suppressor mutants, called nobiro, for rescued root growth. We focused here on nobiro6, which is defective in the general transcription factor component TBP-ASSOCIATED FACTOR 12b (TAF12b). The expression of hundreds of genes, including the bZIP60-UPR regulon, was induced in the bz1728 mutant, but these inductions were markedly attenuated in the bz1728nobiro6 mutant. In view of this, we assigned transcriptional cofactor activity via physical interaction with bZIP60 to NOBIRO6/TAF12b. The single nobiro6/taf12b mutant also showed an altered sensitivity to endoplasmic reticulum stress for both UPR and root growth responses, demonstrating that NOBIRO6/TAF12b contributes to environment-responsive root growth control through UPR.

Original languageEnglish
Article numbere2120219119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number6
DOIs
Publication statusPublished - 8 Feb 2022

Keywords

  • General transcription factor
  • Root growth
  • Stress-responsive growth regulation
  • Unfolded protein response

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