TY - JOUR
T1 - Analysis of human IgE epitope of Der f 2 with anti-Der f 2 mouse monoclonal antibodies
AU - Nishiyama, Chiharu
AU - Hatanaka, Hideki
AU - Ichikawa, Saori
AU - Fukada, Minako
AU - Akagawa-Chihara, Midori
AU - Yuuki, Toshifumi
AU - Yokota, Toyokazu
AU - Inagaki, Fuyuhiko
AU - Okumura, Yasushi
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/1
Y1 - 1999/1
N2 - Der f 2 is one of the major mite allergens recognized by human IgE antibodies of allergic patients, using five anti-Der f 2 mouse monoclonal antibodies, human IgE epitopes of Der f 2 were analyzed. Among them, two monoclonal antibodies 15E11 and 13A4 inhibited the binding between Der f 2 and human IgE antibodies. To determine major IgE epitopes of Der f 2, epitopes for the monoclonal IgG antibodies were analyzed using 43 single site Der f 2 mutants constructed by site-directed mutagenesis. Binding ability of 13A4 and 15E11 was decreased by the amino acid replacement around the C- terminus, and around 73rd, respectively. These results suggest that the C- terminal portion and the central portion around 73rd of Der f 2 were recognized by human IgE antibodies as major epitopes. The location of the putative IgE epitopes on 3-D structure of Der f 2 is also discussed.
AB - Der f 2 is one of the major mite allergens recognized by human IgE antibodies of allergic patients, using five anti-Der f 2 mouse monoclonal antibodies, human IgE epitopes of Der f 2 were analyzed. Among them, two monoclonal antibodies 15E11 and 13A4 inhibited the binding between Der f 2 and human IgE antibodies. To determine major IgE epitopes of Der f 2, epitopes for the monoclonal IgG antibodies were analyzed using 43 single site Der f 2 mutants constructed by site-directed mutagenesis. Binding ability of 13A4 and 15E11 was decreased by the amino acid replacement around the C- terminus, and around 73rd, respectively. These results suggest that the C- terminal portion and the central portion around 73rd of Der f 2 were recognized by human IgE antibodies as major epitopes. The location of the putative IgE epitopes on 3-D structure of Der f 2 is also discussed.
KW - 3-D structure
KW - Der f 2
KW - Dermatophagoides farinae
KW - IgE-epitope
KW - Mite allergen
KW - Site-directed mutagenesis
UR - http://www.scopus.com/inward/record.url?scp=0032977144&partnerID=8YFLogxK
U2 - 10.1016/S0161-5890(98)00112-6
DO - 10.1016/S0161-5890(98)00112-6
M3 - Article
C2 - 10369420
AN - SCOPUS:0032977144
SN - 0161-5890
VL - 36
SP - 53
EP - 60
JO - Molecular Immunology
JF - Molecular Immunology
IS - 1
ER -