Analyses of cell behavior onto micropatterned hydrophilic/hydrophobic surfaces

Yukiko Tsuda; Akihiko Kikuchi; Jun Kobayashi; Masayuki Yamato; Guoping Chen; Teruo Okano

Analyses of cell behavior onto micropatterned hydrophilic/hydrophobic surfaces

Yukiko Tsuda, Akihiko Kikuchi, Jun Kobayashi, Masayuki Yamato, Guoping Chen, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

Introduction of capillary blood vessels is important to reconstruct complicated tissues and organs. We focused on microprocessing technique for possible construction of capillary blood vessels in vitro. In this study, hydrophilic poly(acrylamide)(PAAm) was immobilized at 100 μm ×100 μm with periodic lattice of 10 μm-width onto glass surfaces utilizing a modified liquid crystal display (LCD) projector and photolithography technique. PAAm pattern formation was confirmed by adsorption of FITC labeled BSA. Seeded endothelial cells adhered and extended only onto 10 μm-lattice glass domains but not onto hydrophilic PAAm domains, indicating successful and selective cell adhesion.

Original language	English
Pages	2238
Number of pages	1
Publication status	Published - 2005
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 25 May 2005 → 27 May 2005

Conference

Conference	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	25/05/05 → 27/05/05

Keywords

Hydrophilicity
LCD projector
Micropatterned surface
Photolithography
Poly(acrylamide)

Cite this

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title = "Analyses of cell behavior onto micropatterned hydrophilic/hydrophobic surfaces",

abstract = "Introduction of capillary blood vessels is important to reconstruct complicated tissues and organs. We focused on microprocessing technique for possible construction of capillary blood vessels in vitro. In this study, hydrophilic poly(acrylamide)(PAAm) was immobilized at 100 μm ×100 μm with periodic lattice of 10 μm-width onto glass surfaces utilizing a modified liquid crystal display (LCD) projector and photolithography technique. PAAm pattern formation was confirmed by adsorption of FITC labeled BSA. Seeded endothelial cells adhered and extended only onto 10 μm-lattice glass domains but not onto hydrophilic PAAm domains, indicating successful and selective cell adhesion.",

keywords = "Hydrophilicity, LCD projector, Micropatterned surface, Photolithography, Poly(acrylamide)",

author = "Yukiko Tsuda and Akihiko Kikuchi and Jun Kobayashi and Masayuki Yamato and Guoping Chen and Teruo Okano",

year = "2005",

language = "English",

pages = "2238",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Analyses of cell behavior onto micropatterned hydrophilic/hydrophobic surfaces

AU - Tsuda, Yukiko

AU - Kikuchi, Akihiko

AU - Kobayashi, Jun

AU - Yamato, Masayuki

AU - Chen, Guoping

AU - Okano, Teruo

PY - 2005

Y1 - 2005

N2 - Introduction of capillary blood vessels is important to reconstruct complicated tissues and organs. We focused on microprocessing technique for possible construction of capillary blood vessels in vitro. In this study, hydrophilic poly(acrylamide)(PAAm) was immobilized at 100 μm ×100 μm with periodic lattice of 10 μm-width onto glass surfaces utilizing a modified liquid crystal display (LCD) projector and photolithography technique. PAAm pattern formation was confirmed by adsorption of FITC labeled BSA. Seeded endothelial cells adhered and extended only onto 10 μm-lattice glass domains but not onto hydrophilic PAAm domains, indicating successful and selective cell adhesion.

AB - Introduction of capillary blood vessels is important to reconstruct complicated tissues and organs. We focused on microprocessing technique for possible construction of capillary blood vessels in vitro. In this study, hydrophilic poly(acrylamide)(PAAm) was immobilized at 100 μm ×100 μm with periodic lattice of 10 μm-width onto glass surfaces utilizing a modified liquid crystal display (LCD) projector and photolithography technique. PAAm pattern formation was confirmed by adsorption of FITC labeled BSA. Seeded endothelial cells adhered and extended only onto 10 μm-lattice glass domains but not onto hydrophilic PAAm domains, indicating successful and selective cell adhesion.

KW - Hydrophilicity

KW - LCD projector

KW - Micropatterned surface

KW - Photolithography

KW - Poly(acrylamide)

UR - http://www.scopus.com/inward/record.url?scp=33645643075&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645643075

SP - 2238

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Analyses of cell behavior onto micropatterned hydrophilic/hydrophobic surfaces

Abstract

Conference

Keywords

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