An efficient method to quantitatively detect competitive adsorption of DNA on single-walled carbon nanotube surfaces

Yuhei Horii, Shusuke Ohura, Kazuo Umemura

Research output: Contribution to journalArticle

Abstract

In this study, we quantitatively detected adsorption and desorption of DNA molecules that competed with sodium cholate (SC) molecules on single-walled carbon nanotubes (SWNTs) by fluorescence spectroscopy. In previous studies, competitive adsorption and/or replacement were studied based on techniques such as near-infrared (NIR) absorbance and photoluminescence (PL) spectroscopy of SWNTs. In those studies, adsorption of organic molecules was detected as spectral changes in SWNTs, but not in organic molecules. In this study, we employed fluorescent-labeled DNA (Fc-DNA) to detect competitive adsorption through quenching of fluorescent dyes that were attached to DNA molecules. Through this approach, the adsorption behaviors of DNA molecules could be directly determined. Hence, we found that Fc-DNA molecules adsorbed on SWNT surfaces that were pre-wrapped with SC when the SC concentration was reduced. However, when SC concentrations recovered after three days of incubation, detachment of Fc-DNA molecules was observed. In addition, our method could be applied to evaluate the adsorption of fluorescent dyes on SWNT surfaces instead of DNA molecules. Hence, our method is effective in studying competitive adsorption of organic molecules on SWNT surfaces. The obtained information is complementary to that obtained from NIR spectroscopy of SWNTs.

Original languageEnglish
Article number113776
JournalAnalytical Biochemistry
Volume601
DOIs
Publication statusPublished - 15 Jul 2020

Keywords

  • Adsorption
  • Desorption
  • DNA
  • Fluorescence spectroscopy
  • Single-walled carbon nanotubes (SWNT)
  • Surfactant

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