Silver turns up the A-C: In the presence of Ag I ions, a DNA polymerase incorporated deoxyadenosine (from dATP) at the site opposite cytosine in the template strand to afford the full-length product (see scheme), meaning that DNA polymerases prefer a C-Ag I-A base pair to the more thermodynamically stable C-Ag I-C base pair.
- DNA polymerases
- DNA recognition
- metal ion-mediated base pairs
- primer extension
- silver ions