A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

Kanako Miyano, Katsuya Ohbuchi, Yuka Sudo, Kouichiro Minami, Toru Yokoyama, Masahiro Yamamoto, Miaki Uzu, Miki Nonaka, Seiji Shiraishi, Hiroaki Murata, Yoshikazu Higami, Yasuhito Uezono

Research output: Contribution to journalArticle

Abstract

Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.

Original languageEnglish
JournalJournal of Pharmacological Sciences
DOIs
Publication statusAccepted/In press - 1 Jan 2020

Keywords

  • CellKey™ system
  • Cellular dielectric spectroscopy
  • TRPV1

Fingerprint Dive into the research topics of 'A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy'. Together they form a unique fingerprint.

  • Cite this

    Miyano, K., Ohbuchi, K., Sudo, Y., Minami, K., Yokoyama, T., Yamamoto, M., Uzu, M., Nonaka, M., Shiraishi, S., Murata, H., Higami, Y., & Uezono, Y. (Accepted/In press). A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy. Journal of Pharmacological Sciences. https://doi.org/10.1016/j.jphs.2020.05.001