2a peptides contribute to the co-expression of proteins for imaging and genome editing

Mio K. Shibuta, Megumi Matsuoka, Sachihiro Matsunaga

Research output: Contribution to journalArticle

Abstract

2A self-cleaving peptides (2A peptides), originally identified in a short oligopeptide sequence of foot and mouth disease RNA virus, consist of short amino acids that specify ribosome skipping. They enable the expression of multiple proteins within a single open reading frame through a co-translational cleavage event. The 2A-mediated co-expression system can overcome the problem of uneven expression of different proteins, and contributes to the development of leading technology for imaging and genome editing. It may also enable the evaluation of the expression levels of proteins through comparison of the intensity of their fluorescence. This review provides a recent overview of the 2A-mediated co-expression system and introduces several optimizations to improve cleavage efficiency for practical use.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalCYTOLOGIA
Volume84
Issue number2
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

protein synthesis
image analysis
peptides
oligopeptides
Peptides
genome
foot-and-mouth disease
ribosomes
open reading frames
Foot-and-Mouth Disease Virus
Oligopeptides
Proteins
proteins
RNA Viruses
fluorescence
Ribosomes
Open Reading Frames
amino acids
Fluorescence
Technology

Keywords

  • 2A peptide
  • Co-expression
  • Genome editing
  • Imaging
  • Multi-color analysis
  • Ribosome skipping

Cite this

Shibuta, Mio K. ; Matsuoka, Megumi ; Matsunaga, Sachihiro. / 2a peptides contribute to the co-expression of proteins for imaging and genome editing. In: CYTOLOGIA. 2019 ; Vol. 84, No. 2. pp. 107-111.
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2a peptides contribute to the co-expression of proteins for imaging and genome editing. / Shibuta, Mio K.; Matsuoka, Megumi; Matsunaga, Sachihiro.

In: CYTOLOGIA, Vol. 84, No. 2, 01.01.2019, p. 107-111.

Research output: Contribution to journalArticle

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